期刊
BIOCONTROL SCIENCE AND TECHNOLOGY
卷 25, 期 11, 页码 1285-1298出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09583157.2015.1049977
关键词
entomopathogenic fungus; Aspergillus oryzae; locust; biological control; bioassay; phylogenetic analysis
资金
- Special Fund for Agro-scientific Research in the Public Interest [200903021]
A novel entomopathogenic fungus of Locusta migratoria was identified as Aspergillus oryzae using a comparative sequence analysis of the internal transcribed spacer regions, aflatoxin B1 detection and morphological analysis. The fungus isolated from a dead locust collected in northwestern China was found to be pathogenic to the insect. Phylogenetic experiments revealed a 99% similarity between the fungus and those of three species, A. oryzae, Aspergillus flavus and Aspergillus parvisclerotigenus which are in the same branch of the Flavi section of the genus Aspergillus. Tests to detect aflatoxin B1 demonstrated that this fungus is a non-aflatoxin B1 producer, unlike A. parvisclerotigenus. Furthermore, morphological comparison with A. oryzae and A. flavus revealed that Aspergillus sp. XJ-1 belongs to A. oryzae, and named as A. oryzae XJ-1. The results of bioassays against third-instar locusts showed that mortality was dose-dependent and its median lethal concentrations were 3.3 x 10(8), 1.7 x 10(7) and 7.2 x 10(6) conidia/ml on the 10th-, 13th- and 15th-day post-inoculation. Therefore, the A. oryzae XJ-1 may have biocontrol potential against locusts.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据