期刊
ADVANCED SYNTHESIS & CATALYSIS
卷 358, 期 20, 页码 3298-3306出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adsc.201600682
关键词
amino acids; asymmetric synthesis; biocatalysis; enzyme catalysis; oxidoreductases
资金
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Glaxo-SmithKline (GSK) under the Strategic Longer and Larger (sLoLa) grant initiative [BB/K00199X/1]
- BBSRC [BB/M017702/1]
- Royal Society
- BBSRC [BB/M017702/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M017702/1] Funding Source: researchfish
A practical and efficient biocatalytic synthesis of aromatic D-amino acids has been developed, based on the reductive amination of the corresponding a-keto acids via a recombinant whole cell system composed of an engineered dehydrogenase and cofactor recycling apparatus. The reaction was shown to give excellent enantioselectivity (>= 98%) and good yields at the preparative scale across a broad range of substrates. Additionally, the structure of the variant enzyme was solved to allow rationalisation of the observed reaction rates. The engineered whole cell catalyst was also used to mediate the production of D-phenylalanine derivatives from racemic mixtures and cheaper l-amino acids by combining it with an enantiocomplementary deaminase.
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