期刊
BIOCONJUGATE CHEMISTRY
卷 26, 期 6, 页码 963-974出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.5b00167
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资金
- National Institutes of Health (NIH) [S10 OD016237]
- Molecular Imaging Center - NCI [P50CA094056]
- NCI [R01 CA171651]
- NIBIB [R01 EB008111]
- Imaging Sciences Pathway graduate student fellowship, at Washington University in St. Louis [NIH T32 EB014855]
Visualization of biological processes and pathologic conditions at the cellular and tissue levels largely relies on the use of fluorescence intensity signals from fluorophores or their bioconjugates. To overcome the concentration dependency of intensity measurements, evaluate subtle molecular interactions, and determine biochemical status of intracellular or extracellular microenvironments, fluorescence lifetime (FLT) imaging has emerged as a reliable imaging method complementary to intensity measurements. Driven by a wide variety of dyes exhibiting stable or environment-responsive FLTs, information multiplexing can be readily accomplished without the need for ratiometric spectral imaging. With knowledge of the fluorescent states of the molecules, it is entirely possible to predict the functional status of biomolecules or microevironment of cells. Whereas the use of FLT spectroscopy and microscopy in biological studies is now well-established, in vivo imaging of biological processes based on FLT imaging techniques is still evolving. This review summarizes recent advances in the application of the FLT of molecular probes for imaging cells and small animal models of human diseases. It also highlights some challenges that continue to limit the full realization of the potential of using FLT molecular probes to address diverse biological problems and outlines areas of potential high impact in the future.
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