期刊
BIOCONJUGATE CHEMISTRY
卷 26, 期 6, 页码 1017-1020出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.5b00282
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This Communication describes a bioconjugation method for the generation of enzyme microarrays on surfaces using photochemical thiol-ene chemistry in combination with microcontact printing. Glucose oxidase and lactase were readily immobilized (i.e., printing time 2 min) on alkene terminated self-assembled monolayers on glass as demonstrated by X-ray photoelectron spectroscopy and fluorescence microscopy. Furthermore, the activity of both immobilized enzymes was confirmed in single enzyme as well as cascade transformations.
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