期刊
BIOCONJUGATE CHEMISTRY
卷 26, 期 8, 页码 1672-1677出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.5b00266
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- JSPS Institutional Program for Young Researcher Overseas Visits
- Grants-in-Aid for Scientific Research [15K13781, 26289310] Funding Source: KAKEN
Protein-based nanoparticles are attractive carriers for drug delivery because they are biodegradable and can be genetically designed. Moreover, modification of protein-based nanoparticles with cell-specific ligands allows for active targeting abilities. Previously, we developed protein nanoparticles comprising genetically engineered elastin-like polypeptides (ELPs) with fused polyaspartic acid tails (ELP-D). Epidermal growth factor (EGF) was displayed on the surface of the ELP-D nanoparticles via genetic design to allow for active cell-targeting abilities. Herein, we focused on the coiled-coil structural motif as a means for noncovalent tethering of growth factor to ELP-D. Specifically, two peptides known to form a heterodimer via a coiled-coil structural motif were fused to ELP-D and single-chain vascular endothelial growth factor (scVEGF(121)), to facilitate noncovalent tethering upon formation of the heterodimer coiled-coil structure. Drug-loaded growth factor-tethered ELP-Ds were found to be effective against cancer cells by provoking cell apoptosis. These results demonstrate that tethering growth factor to protein nanoparticles through coiled-coil formation yields a promising biomaterial candidate for targeted drug delivery.
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