期刊
VIROLOGICA SINICA
卷 33, 期 5, 页码 410-417出版社
SPRINGER
DOI: 10.1007/s12250-018-0051-0
关键词
Middle East Respiratory Syndrome Coronavirus (MERS-CoV); Camel; Human; Pakistan
类别
资金
- International Cooperation on Key Technologies of Biosafety [153B42KYSB20170004]
- External Cooperation Program of CAS [153211KYSB20160001]
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in Pakistan, nasal (n=776) and serum (n=1050) samples were collected from camels between November 2015 and February 2018. Samples were collected from animal markets, free-roaming herds and abattoirs. An in-house ELISA was developed to detect IgG against MERS-CoV. A total of 794 camels were found seropositive for MERS-CoV. Prevalence increased with the age and the highest seroprevalence was recorded in camels aged>10years (81.37%) followed by those aged 3.1-10years (78.65%) and3years (58.19%). Higher prevalence was observed in female (78.13%) as compared to male (70.70%). Of the camel nasal swabs, 22 were found to be positive by RT-qPCR though with high Ct values. Moreover, 2,409 human serum samples were also collected from four provinces of Pakistan during 2016-2017. Among the sampled population, 840 humans were camel herders. Although we found a high rate of MERS-CoV antibody positive dromedaries (75.62%) in Pakistan, no neutralizing antibodies were detected in humans with and without contact to camels.
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