期刊
ADVANCED FUNCTIONAL MATERIALS
卷 26, 期 43, 页码 7824-7835出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201602600
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资金
- Department of Defense/Air Force Office of Scientific Research [FA95501310192]
- National Institutes of Health/National Cancer Institute [U54CA151880, R01CA167041]
- Ryan Family
- Malkin Family
- Driskill Family
- Chicago Baseball Charities Cancer Fellowship
- T32 Carcinogenesis training grant [T32CA09560]
- NCI CCSG [P30 CA060553]
Efficient systemic administration of therapeutic short interfering RNA (siRNA) is challenging. High-density lipoproteins (HDLs) are natural in vivo RNA delivery vehicles. Specifically, native HDLs: 1) load single-stranded RNA; 2) are anionic, which requires charge reconciliation between the RNA and HDL, and 3) actively target scavenger receptor type B-1 (SR-B1) to deliver RNA. Emphasizing these particular parameters, templated lipoprotein particles (TLP), mimics of spherical HDLs, are employed and are self-assembled with single-stranded complements of, presumably, any highly unmodified siRNA duplex pair after formulation with a cationic lipid. Resulting siRNA templated lipoprotein particles (siRNA-TLP) are anionic and tunable with regard to RNA assembly and function. Data demonstrate that the siRNA-TLPs actively target SR-B1 to potently reduce androgen receptor and enhancer of zeste homolog 2 proteins in multiple cancer cell lines. Systemic administration of siRNA-TLPs demonstrated no off-target toxicity and significantly reduced the growth of prostate cancer xenografts. Thus, native HDLs inspired the synthesis of a hybrid siRNA delivery vehicle that can modularly load single-stranded RNA complements after charge reconciliation with a cationic lipid, and that function due to active targeting of SR-B1.
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