期刊
PHARMACEUTICS
卷 10, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/pharmaceutics10040229
关键词
flow-focusing microfluidics; cell-laden microgel; mechanics; tumor spheroid; co-culture
资金
- 2018 Research Fund of UNIST (Ulsan National Institute of Science and Technology) [1.180042.01]
- Civil-Military Technology Cooperation Program [15-CM-SS-03]
- Bio & Medical Technology Development Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2017M3A9C6033875]
- Ministry of Science & ICT (MSIT), Republic of Korea [2018U-K BRAND 육성사업] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2017M3A9C6033875] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Tumor spheroids are considered a valuable three dimensional (3D) tissue model to study various aspects of tumor physiology for biomedical applications such as tissue engineering and drug screening as well as basic scientific endeavors, as several cell types can efficiently form spheroids by themselves in both suspension and adherent cell cultures. However, it is more desirable to utilize a 3D scaffold with tunable properties to create more physiologically relevant tumor spheroids as well as optimize their formation. In this study, bio-active spherical microgels supporting 3D cell culture are fabricated by a flow-focusing microfluidic device. Uniform-sized aqueous droplets of gel precursor solution dispersed with cells generated by the microfluidic device are photo-cross-linked to fabricate cell-laden microgels. Their mechanical properties are controlled by the concentration of gel-forming polymer. Using breast adenocarcinoma cells, MCF-7, the effect of mechanical properties of microgels on their proliferation and the eventual spheroid formation was explored. Furthermore, the tumor cells are co-cultured with macrophages of fibroblasts, which are known to play a prominent role in tumor physiology, within the microgels to explore their role in spheroid formation. Taken together, the results from this study provide the design strategy for creating tumor spheroids utilizing mechanically-tunable microgels as 3D cell culture platform.
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