期刊
CELLS
卷 8, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/cells8010048
关键词
nanobodies; super-resolution microscopy; multi-color imaging; fluorescent proteins; microfluidics; DNA-PAINT; molecular localization; single domain antibodies (sdAb); multiplexing; linkage error
类别
资金
- Deutsche Forschungsgemeinschaft (DFG) through the Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)
- DFG [SFB 860, SFB 803]
- Deutsche Forschungsgemeinschaft (DFG)
DNA point accumulation for imaging in nanoscale topography (PAINT) is a rapidly developing fluorescence super-resolution technique, which allows for reaching spatial resolutions below 10 nm. It also enables the imaging of multiple targets in the same sample. However, using DNA-PAINT to observe cellular structures at such resolution remains challenging. Antibodies, which are commonly used for this purpose, lead to a displacement between the target protein and the reporting fluorophore of 20-25 nm, thus limiting the resolving power. Here, we used nanobodies to minimize this linkage error to similar to 4 nm. We demonstrate multiplexed imaging by using three nanobodies, each able to bind to a different family of fluorescent proteins. We couple the nanobodies with single DNA strands via a straight forward and stoichiometric chemical conjugation. Additionally, we built a versatile computer-controlled microfluidic setup to enable multiplexed DNA-PAINT in an efficient manner. As a proof of principle, we labeled and imaged proteins on mitochondria, the Golgi apparatus, and chromatin. We obtained super-resolved images of the three targets with 20 nm resolution, and within only 35 minutes acquisition time.
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