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Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium

期刊

CELLS
卷 8, 期 1, 页码 -

出版社

MDPI
DOI: 10.3390/cells8010046

关键词

CRISPR/Cas9; CRISPR vector; Dictyostelium; genome editing; tRNA-based expression; Amoebozoa

资金

  1. JSPS KAKENHI [17K07416, 16KT0177]
  2. Grants-in-Aid for Scientific Research [17K07416, 16KT0177] Funding Source: KAKEN

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In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including Dictyostelium, enabling rapid high-fidelity modification of endogenous genes. Here we reviewed recent progress in genome editing in Dictyostelium and summarised useful CRISPR vectors that express sgRNA and Cas9, including several microorganisms. Using these vectors, precise genome modifications can be achieved within 2-3 weeks, beginning with the design of the target sequence. Finally, we discussed future perspectives on the use of CRISPR/Cas9-mediated genome editing in Dictyostelium.

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