期刊
SCIENCE ADVANCES
卷 5, 期 1, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.aau8355
关键词
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资金
- National Key Research and Development Program of China [2017YFA0700501]
- National Nature Science Foundation of China [61860206009, 81870934, 31571002, 81701354, 91749209]
- Science Fund for Creative Research Group of China [61721092]
- China Postdoctoral Science Foundation [2017M612463, 2018T110772]
- Fundamental Research Funds for the Central Universities, HUST [2018KFYXKJC026]
- Director Fund of WNLO
Various optical clearing methods have emerged as powerful tools for deep biological imaging. Organic solvent-based clearing methods, such as three-dimensional imaging of solvent-cleared organs (3DISCO), present the advantages of high clearing efficiency and size reduction for panoptic imaging of large samples such as whole organs and even whole bodies. However, 3DISCO results in a rapid quenching of endogenous fluorescence, which has impeded its application. Here, we propose an advanced method named FDISCO to overcome this limitation. FDISCO can effectively preserve the fluorescence of various fluorescent probes and can achieve a long storage time of months while retaining potent clearing capability. We used FDISCO for high-resolution imaging and reconstruction of neuronal and vascular networks. Moreover, FDISCO is compatible with labeling by multiple viruses and enables fine visualization of neurons with weak fluorescence labeling in the whole brain. FDISCO represents an effective alternative to the three-dimensional mapping of whole organs and can be extensively used in biomedical studies.
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