期刊
ACS SENSORS
卷 4, 期 1, 页码 110-117出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.8b01000
关键词
microRNA; catalytic hairpin assembly; DNAzyme; intracellular imaging; signal amplification
资金
- Natural Science Foundation of China [21874103, 21503151, 81602610]
- National Basic Research Program of China (973 Program) [2015CB932601]
- Jiangsu Provincial NSFC [BK20161248, BK20160381]
- Wuhan Youth Science and Technology Plan [2016070204010131]
- Fundamental Research Funds for the Central Universities [2042018kf0210]
Abnormal microRNAs (miRNAs) expression is demonstrated to associate with various important biological processes, including tumorigenesis, metastasis, and progression. Given the low miRNA expression at the earlier stage of diseases, its amplified detection still requires more efforts. Inspired by the two stage arithmetic amplifier of electric devices, we reported an autonomous and catalytic assembly of DNAzyme strategy by integrating a DNAzyme biocatalyst and catalytic hairpin assembly (CHA) circuit. Here the catalytically inactive DNAzyme subunits were respectively grafted into these metastable CHA hairpin reactants that were kinetically impeded without false cross hybridizations. The target catalyzed the nonenzymatic CHA-mediated successive assembly of dumbbell-like bis-DNAzyme nanostructures, leading to the efficient DNAzyme-mediated cleavage of fluorophore/quencher-modified substrate and to the generation of an amplified fluorescence signal. The present CHA-DNAzyme amplifier can be employed as a versatile and general sensing platform for analyzing other analytes (e.g., miRNA) by introducing a sensing module into the present system. Moreover, the homogeneous CHA-DNAzyme method could realize the sensitive intracellular miRNA imaging in living cells, which is attributed to the inherently synergistic amplification property between DNAzyme and CHA reactions. Given the attractive analytical features of the autonomous CHA-DNAzyme system, the present strategy shows great promise for analyzing additional different analytes in clinical research fields.
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