期刊
CANCER MANAGEMENT AND RESEARCH
卷 10, 期 -, 页码 5825-5838出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/CMAR.S180334
关键词
12-HETE; ILK; apoptosis; NF-kappa B; ovarian cancer
类别
资金
- National Natural Science Youth Foundation of China [81401502]
- Nature Scientific Foundation of Heilongjiang Province [H2018020]
- Heilongjiang Postdoctoral Foundation and Health Department Foundation of Heilongjiang Province [2010-097]
Background: The dysfunction of cell apoptosis is an important event in the progression of cancer, and the growth of cancer cells is negatively regulated by cell apoptosis. In different types of cancers, inhibition of cellular apoptosis is often observed in the cancerous tissue, and increased resistance to apoptosis is a hallmark of cancer. Although previous studies have shown that 12-lipoxygenase (12-LOX)/12-hydroxyeicosatetraenoic acid (12-HETE) is activated and upregulated in different types of cancers, the consequences of 12-LoX/12-HETE upregulation and its precise roles in the survival of ovarian carcinoma cells are still unknown. Methods: MTT assays, caspase activity assays, lactate dehydrogenase (LDH) assays, and Western blot analysis were the methods used in this study. Results: In our study, we found that 12-HETE, a major metabolic product of arachidonic acid using 12-LOX catalysis, inhibited cell apoptosis in a dose-dependent manner and that the effects of 12-HETE on cell apoptosis were mediated by the integrin-linked kinase (ILK) pathway. Moreover, the downstream target of 12-HETE-activated ILK was nuclear factor kappa-B (NF-kappa B) in ovarian carcinoma. The inhibitory effects of 12-HETE on cell apoptosis were attenuated by the inhibition of the NF-kappa B pathway. Conclusion: These results indicate that 12-HETE participates in the inhibition of cell apoptosis by activating the ILK/NF-kappa B pathway, implying an important underlying mechanism that promotes the survival of ovarian cancer cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据