期刊
BIOCHIMIE
卷 118, 期 -, 页码 264-269出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2015.06.015
关键词
Archaea; Hyperthermophile; Endonuclease; DNA repair; Thermococcale
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [21113005, 23310152, 26242075]
- Institute for fermentation, Osaka (IFO)
- [26-9354]
Base deamination is a typical form of DNA damage, and it must be repaired quickly to maintain the genome integrity of living organisms. Endonuclease Q (EndoQ), recently found in the hyperthermophilic archaea, is an enzyme that cleaves the phosphodiester bond 5' from the damaged nucleotide in the DNA strand, and may primarily function to start the repair process for the damaged bases. Endonuclease V (EndoV) also hydrolyzes the second phosphodiester bond 3' from the damaged nucleotide, although the hyperthermophilic archaeal EndoV is a strictly hypoxanthine-specific endonuclease. To understand the relationships of the EndoQ and EndoV functions in hyperthermophilic archaea, we analyzed their interactions in hypoxanthine repair. EndoQ and EndoV do not directly interact with each other in either the presence or absence of DNA. However, EndoQ and EndoV individually worked on deoxyinosine (dl)-containing DNA at each cleavage site. EndoQ has higher affinity to dl-containing DNA than EndoV, and cells produce higher amounts of EndoQ as compared to EndoV. These data support the proposal that EndoQ primarily functions for, at least, dl-containing DNA. (C) 2015 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据