期刊
PROBIOTICS AND ANTIMICROBIAL PROTEINS
卷 11, 期 4, 页码 1340-1347出版社
SPRINGER
DOI: 10.1007/s12602-018-9475-y
关键词
Probiotics; Encapsulation; Buttermilk proteins; Agave fructans
资金
- National Council of Science and Technology of Mexico, CONACyT [CB169358]
Lactobacillus rhamnosus GG (L. rhamnosus GG) cells were encapsulated in buttermilk proteins by spray drying, alone (E), or with Agave tequilana fructans (CEF). Buttermilk proteins acted as a thermo-protector for the probiotic cells undergoing the spray-dried process. The addition of Agave fructans in CEF microcapsules significantly enhanced storage stability and survival to in vitro simulated gastrointestinal conditions, compared to E capsules. After 14 days storage at - 20 degrees C, the number of living cells in CEF microcapsules was in the order of 7.7 log CFU center dot mL(-1) and the survivability in simulated gastrointestinal environment was 73.23%. Spray-dried microparticles were cultured in goat milk to study biomass production. Agave fructans offered a favorable microenvironment and better growth substrate. The population of CEF viable cells reached 1.08 +/- 0.02 x 10(10) CFU center dot mL(-1) after 18 h of fermentation. In contrast, the population of E viable cells were 3.0 +/- 0.01 x 10(9) CFU center dot mL(-1). The generation time of CEF, L. rhamnosus GG was 15% faster than E, L. rhamnosus GG. Encapsulation with buttermilk proteins in the presence of Agave fructans by spray drying could be suitable for preservation of probiotic powders and may be for a more effective application of probiotics in goat dairy products.
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