期刊
FRONTIERS IN IMMUNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2018.02736
关键词
immunometabolism; mTOR; naive T-cells; priming; CD8(+) T-lymphocytes
类别
资金
- ANR [ANR-14-CE14-0030-01]
- Universita ItaloFrancese/Universite FrancoItalienne [G10-718, 39582TJ]
- Wellcome Trust [100326/Z/12/Z]
- Agence Nationale de la Recherche (ANR) [ANR-14-CE14-0030] Funding Source: Agence Nationale de la Recherche (ANR)
- Wellcome Trust [100326/Z/12/Z] Funding Source: researchfish
Background: Characterization of the intracellular biochemical processes that regulate the generation and maintenance of effector and memory CD8(+) T-cells from naive precursors is essential for our understanding of adaptive immune responses and the development of immunotherapies. However, the metabolic determinants of antigen-driven activation and differentiation remain poorly defined, especially in humans. Methods: We used a variety of different approaches, including gene expression profiling and measurements of nutrient flux, to characterize the basal and activation-induced energetic requirements of naive and phenotypically-defined subsets of human memory CD8(+) T-cells. Findings: Profound metabolic differences were apparent as a function of differentiation status, both at rest and in response to stimulation via the T cell receptor (TCR). Of particular note, resting naive CD8(+) T cells were largely quiescent, but rapidly upregulated diverse energetic pathways after ligation of surface-expressed TCRs. Moreover, autophagy and the mechanistic target of rapamycin (mTOR)-dependent glycolytic pathway were identified as critical mediators of antigen-driven priming in the naive CD8(+) T cell pool, the efficiency of which was dampened by the presence of neutral lipids and fatty acids. Interpretation: These observations provide a metabolic roadmap of the CD8(+) T-cell compartment in humans and reveal potentially selective targets for novel immunotherapies.
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