期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1854, 期 6, 页码 559-580出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2014.10.003
关键词
Formalin; FFPE; Tissue; Proteomics; Cancer; Tumour
资金
- Ovarian Cancer Research Foundation (OCRF)
- Bioplatforms Australia
- Government of South Australia
Retrospective proteomic studies, including those which aim to elucidate the molecular mechanisms driving cancer, require the assembly and characterization of substantial patient tissue cohorts. The difficulty of maintaining and accessing native tissue archives has prompted the development of methods to access archives of formalin-fixed tissue. Formalin-fixed tissue archives, complete with patient meta data, have accumulated for decades, presenting an invaluable resource for these retrospective studies. This review presents the current knowledge concerning formalin-fixed tissue, with descriptions of the mechanisms of formalin fixation, protein extraction, top-down proteomics, bottom-up proteomics, quantitative proteomics, phospho- and glycoproteomics as well as imaging mass spectrometry. Particular attention has been given to the inclusion of proteomic investigations of archived tumour tissue. This article is part of a Special Issue entitled: Medical Proteomics. (C) 2014 The Authors. Published by Elsevier B.V.
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