期刊
BONE & JOINT RESEARCH
卷 8, 期 1, 页码 11-18出版社
BRITISH EDITORIAL SOC BONE JOINT SURGERY
DOI: 10.1302/2046-3758.81.BJR-2018-0181.R1
关键词
Tranexeimc acid; Apoptosis; Periarticular tissues; Tendon; Cartilage; Synovium
资金
- Royal College of Surgeons (Edinburgh)
- Arthritis Research UK [21346]
- MRC [MR/R020515/1] Funding Source: UKRI
- Medical Research Council [MR/R020515/1] Funding Source: researchfish
- Versus Arthritis [21346] Funding Source: researchfish
Objectives Tranexamic acid (TXA) is an anti-fibrinolytic medication commonly used to reduce perioperative bleeding. Increasingly, topical administration as an intra-articular injection or perioperative wash is being administered during surgery. Adult soft tissues have a poor regenerative capacity and therefore damage to these tissues can be harmful to the patient. This study investigated the effects of TXA on human periarticular tissues and primary cell cultures using clinically relevant concentrations. Methods Tendon, synovium, and cartilage obtained from routine orthopaedic surgeries were used for ex vivo and in vitro studies using various concentrations of TXA. The in vitro effect of TXA on primary cultured tenocytes, fibroblast-like synoviocytes, and chondrocytes was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MU) cell viability assays, fluorescent microscopy, and multi-protein apoptotic arrays for cell death. Results There was a significant (p < 0.01) increase in cell death within all tissue explants treated with 100 mg/ml TXA. MU assays revealed a significant (p < 0.05) decrease in cell viability in all tissues following treatment with 50 mg/ml or 100 mg/ml of TXA within four hours. There was a significant (p < 0.05) increase in cell apoptosis after one hour of exposure to TXA (100 mg/ml) in all tissues. Conclusion The current study demonstrates that TXA caused significant periarticular tissue toxicity ex vivo and in vitro at commonly used clinical concentrations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据