期刊
ACTA TROPICA
卷 158, 期 -, 页码 43-51出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.actatropica.2016.02.005
关键词
Giardia; Cryptosporidium; Dissemination; Detection; Wastewater; LAMP
资金
- Linksniederrheinische Entwasserungs-Genossenschaft (LINEG), Kamp-Lintfort, Germany
- Laboratory for Medical Parasitology at the University of Cologne
Environmental water samples from the Lower Rhine area in Germany were investigated via immunofluorescence assays (IFAs), nested polymerase chain reaction (nested PCR) and loop-mediated isothermal amplification (LAMP) to detect the presence of Giardia spp. (n =185) and Cryptosporidium spp. (n=227). The samples were concentrated through filtration or flocculation, and oocysts were purified via centrifugation through a sucrose density gradient. For all samples, IFA was performed first, followed by DNA extraction for the nested PCR and LAMP assays. Giardia cysts were detected in 105 samples (56.8%) by IFA, 62 samples (33.5%) by nested PCR and 79 samples (42.7%) by LAMP. Cryptosporidium spp. were detected in 69 samples (30.4%) by IFA, 95 samples (41.9%) by nested PCR and 99 samples (43.6%) by LAMP. According to these results, the three detection methods are complementary for monitoring Giardia and Cryptosporidium in environmental waters. (C) 2016 Elsevier B.V. All rights reserved.
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