期刊
ADVANCED MATERIALS INTERFACES
卷 5, 期 24, 页码 -出版社
WILEY
DOI: 10.1002/admi.201801373
关键词
dielectric barrier discharges; enzyme catalysis; immobilization; plasma chemistry; XPS of proteins
资金
- Italian Ministry for Education, University and Research (MIUR) [PONa3_00369]
- Regione Puglia [51]
A comprehensive study of the interaction of cold atmospheric pressure plasmas (CAPs) with dry enzymes is conducted to identify the experimental conditions that allow preserving enzyme functionality. Glucose oxidase (GOx) dry deposits are exposed to dielectric barrier discharges fed with pure helium, helium-oxygen, and helium-ethylene mixtures. The GOx loses functionality upon exposure to He/O-2 CAP due to dry etching. X-ray photoelectron spectroscopy reveals that, in parallel with the ablation, there are modifications of the chemical structure of the surface of the enzyme deposit that decrease the etching rate and eventually lead to a crust with a strong resistance against the ablation, responsible of process termination. Interestingly, the customary conditions used for plasma-enhanced chemical vapor deposition using helium-ethylene mixtures (applied voltage <1.1 kV(rms) and exposure time <= 10 min) are friendly for GOx. It is therefore possible to immobilize enzymes by overcoating with a plasma-deposited thin film, fully retaining enzyme functionality.
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