期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1854, 期 9, 页码 1101-1112出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2015.01.010
关键词
Iron-sulfur cluster biogenesis; Rrf2 family; Transcription regulation; Protein-DNA interaction
资金
- national funds through Fundacao para a Ciencia e a Tecnologia (FCT)
- EU-FEDER through the Operational Competitiveness Programme-COMPETE [FCOMP-01-0124-FEDER-028116 [PTDC/BBB-BEP/2127/2012], SFRH/BD/66461/2009]
- EU-FEDER through Programa Operacional Regional do Norte (ON.2 - O Novo Norte), under Quadro de Referencia Estrategico Nacional - QREN [NORTE-07-0124-000002]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/66461/2009] Funding Source: FCT
Iron-sulfur clusters are essential cofactors in a myriad of metabolic pathways. Therefore, their biogenesis is tightly regulated across a variety of organisms and environmental conditions. In Gram-negative bacteria, two pathways - ISC and SUF - concur for maintaining intracellular iron-sulfur cluster balance. Recently, the mechanism of iron-sulfur cluster biosynthesis regulation by IscR, an iron-sulfur cluster-containing regulator encoded by the isc operon, was found to be conserved in some Gram-positive bacteria. Belonging to the Rrf2 family of transcriptional regulators, IscR displays a single helix-turn-helix DNA-binding domain but is able to recognize two distinct DNA sequence motifs, switching its specificity upon cluster ligation. This review provides an overview of gene regulation by iron-sulfur cluster-containing sensors, in the light of the recent structural characterization of cluster-less free and DNA-bound IscR, which provided insights into the molecular mechanism of nucleotide sequence recognition and discrimination of this unique transcription factor. This article is part of a Special Issue entitled: Cofactor-dependent proteins: evolution, chemical diversity and bio-applications. (C) 2015 Elsevier B.V. All rights reserved.
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