期刊
STEM CELLS INTERNATIONAL
卷 2018, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2018/9207678
关键词
-
资金
- Stem Cell and Translation National Key Project [2016YFA0101403]
- National Basic Research Program of China [2011CB965103]
- National Natural Science Foundation of China [81371377, 81661130160, 81422014, 81561138004]
- Beijing Municipal Natural Science Foundation [7172055, 5142005]
- Beijing Talents Foundation [2017000021223TD03]
- Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan [CITTCD20180333]
- Beijing Medical System High Level Talent Award [2015-3-063]
- Royal Society-Newton Advanced Fellowship [NA150482]
- Shandong Provincial Natural Science Foundation [2016ZDJS07A09]
- Science and Technology project item in a social development area of Guangdong Province [2014A020212563]
The resident microglial and infiltrating cells from peripheral circulation are involved in the pathological processes of ischemia stroke and may be regulated by mesenchymal stem/stromal cell (MSC) transplantation. The present study is aimed at differentiating the neurotrophic and inflammatory roles played by microglial vs. infiltrating circulation-derived cells in the acute phase in rat ischemic brains and explore the influences of intravenously infused allogeneic MSCs. The ischemic brain injury was induced by distal middle cerebral artery occlusion (dMCAO) in SD rats, with or without MSC infusion in the same day following dMCAO. Circulation-derived infiltrating cells in the brain were identified by Ly6C, a majority of which were monocytes/macrophages. Without MSC transplantation, among the infiltrated Ly6C(+) cells, some were positive for BDNF, IL-1 beta, or TNF-alpha. Following MSC infusion, the overall number of Ly6C(+) infiltrated cells was reduced by 50%. In contrast, the proportions of infiltrated Ly6C(+) cells coexpressing BDNF, IL-1 beta, or TNF-alpha were significantly enhanced. Interestingly, Ly6C(+) cells in the infarct area could produce either neurotrophic factor BDNF or inflammatory cytokines (IL-1 beta or TNF-alpha), but not both. This suggests that the Ly6C(+) cells may constitute heterogeneous populations which react differentially to the microenvironments in the infarct area. The changes in cellular composition in the infarct area may have contributed to the beneficial effect of MSC transplantation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据