期刊
LIGHT-SCIENCE & APPLICATIONS
卷 7, 期 -, 页码 -出版社
CHINESE ACAD SCIENCES, CHANGCHUN INST OPTICS FINE MECHANICS AND PHYSICS
DOI: 10.1038/s41377-018-0111-0
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资金
- University of Dundee
- Scottish Universities Physics Alliance (PaLS initiative)
- European Regional Development Fund [CZ. 02.1.01/0.0/0.0/15 003/0000476]
- John Fell Fund
- BBSRC (TDRF)
- MRC (UK)
- BBSRC [BB/P02730X/1] Funding Source: UKRI
- MRC [G0701061] Funding Source: UKRI
Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system(1-4). Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)(5-7). We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-mu m-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.
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