期刊
JOURNAL OF CHEMISTRY
卷 2019, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2019/5307340
关键词
-
资金
- ERAF project Environment-friendly cultivation of emerging commercial fruit crop Japanese quince-Chaenomeles japonica and waste-free methods of its processing [1.1.1.1/16/A/094]
u Tocopherol separations employing the same Kinetex C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2min. The RP-LC approach could not separate the isomers and . The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption. The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization. The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species.
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