期刊
FRONTIERS IN MICROBIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2018.02533
关键词
cervicovaginal microbiota; 16S rRNA; ITS2; fungi; cervical cancer
类别
资金
- Institutional Development Award (IDeA) from the NIH National Institute of General Medical Sciences [P20 GM103475]
- NIH National Institute on Minority Health and Health Disparities [2U54MD007600, 2U54MD007587, S21MD001830]
- Inter American University
- [U01AI22245]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM103475] Funding Source: NIH RePORTER
- National Institute on Minority Health and Health Disparities [U54MD007600, U54MD007587] Funding Source: NIH RePORTER
The human cervicovaginal microbiota resides at an interface between the host and the environment and may affect susceptibility to disease. Puerto Rican women have high human papillomavirus (HPV) infection and cervical cancer rates. We hypothesized that the population structure of the cervicovaginal bacterial and fungal biota changed with cervical squamous intraepithelial lesions and HPV infections. DNA was extracted from cervix, introitus, and anal sites of 62 patients attending high-risk San Juan clinics. The 16S rRNA V4 region and ITS-2 fungal regions were amplified and sequenced using Illumina technology. HPV genotyping was determined by reverse hybridization with the HPV SPF10-LiPA25 kit. HPV prevalence was 84% of which similar to 44% subjects were infected with high-risk HPV, similar to 35% were co-infected with as many as 9 HPV types and similar to 5% were infected with exclusively low-risk HPV types. HPV diversity did not change with cervical dysplasia. Cervical bacteria were more diverse in patients with CIN3 pre-cancerous lesions. We found enrichment of Atopobium vaginae and Gardnerella vaginalis in patients with CIN3 lesions. We found no significant bacterial biomarkers associated with HPV infections. Fungal diversity was significantly higher in cervical samples with high-risk HPV and introitus samples of patients with Atypical Squamous Cells of Undetermined Significance (ASCUS). Fungal biomarker signatures for vagina and cervix include Sporidiobolaceae and Sacharomyces for ASCUS, and Malassezia for high-risk HPV infections. Our combined data suggests that specific cervicovaginal bacterial and fungal populations are related to the host epithelial microenvironment, and could play roles in cervical dysplasia.
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