期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2018.00391
关键词
Brucella abortus; respiratory infection; innate immunity; IL-1 beta; inflammasomes
资金
- Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCYT) of Argentina [PICT 2013-0170, PICT CABBIO 2014-0601, PICT 2016-1199]
- Universidad de Buenos Aires [UBACYT 20020130100151BA]
- Fundacion Bunge y Born (Infectious Diseases Grant)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [464711/2014-2, 402527/2013-5, 443662/2014-2, 302660/2015-1]
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil [837/15, 00140-16]
- National Institutes of Health [R01 AI116453]
Brucella spp. infection is frequently acquired through contaminated aerosols. The role of interleukin-1 beta (IL-1 beta) in the early pulmonary response to respiratory Brucella infection is unknown. As shown here, IL-1b levels in lung homogenates and bronchoalveolar lavage fluid (BALF) of mice intratracheally inoculated with B. abortus were increased at 3 and 7 days p.i. At 7 days p.i., pulmonary CFU numbers were higher in IL-1 receptor (IL-1R) knockout (KO) mice than in wild type (WT) mice. At different times p.i. CFU in lungs and BALF were higher in mice lacking some inflammasome components (caspase-1, AIM2, NLRP3) than in WT mice. At 2 days p.i. pulmonary levels of IL-1 beta and CXCL1 (neutrophils chemoattractant) were lower in caspase-1/11 KO mice. At day 3 p.i., neutrophils counts in BALF were lower in caspase-1/11 KO mice than in WT mice. During in vitro infections, IL-1 beta secretion was lower in alveolar macrophages from caspase-1/11, NLRP3 or AIM2 KO mice than in WT controls. Similarly, IL-1 beta production by B. abortus-infected alveolar epithelial cells was reduced by pretreatment with a specific caspase-1 inhibitor. This study shows that IL-1R, probably through IL-1 beta action, and the NLRP3 and AIM2 inflammasomes are involved in pulmonary innate immune protective mechanisms against respiratory B. abortus infection.
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