期刊
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷 6, 期 11, 页码 13696-13705出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.8b01089
关键词
Pulsed electric field; Protein concentrate; Macroalgae; Ulva; Antioxidant; Seaweed
资金
- Israel Ministry of Health, Fund for Medical Studies and Infrastructures Development [3-12788]
- Israel Ministry of Science [3-13572]
- Israel Ministry of Economy [4268]
- German Helmholtz Research Program on Renewable Energies
With decreasing available land and fresh-water resources, the oceans become attractive alternatives for the production of valuable biomass, comparable to terrestrial crops. Seaweed cultivation for food, chemicals, and fuels is already under intensive development, yet efficient technologies for separation of major components are still missing. We report a food-grade process for the extraction of proteins from a green macroalga, Ulva sp., using high-voltage pulsed electric field (PEF) cell-membrane permeabilization, coupled with mechanical pressing to separate liquid and solid phases. We showed that a PEF treatment, at 247 kJ/kg fresh Ulva, delivered through 50 pulses of 50 kV, applied at a 70.3 mm electrode gap on the 140 g fresh weight of Ulva sp., resulted in an similar to 7-fold increase in the total protein extraction yield compared to extraction by osmotic shock. The PEF extract of 20% protein content showed 10-20 times higher antioxidant capacity than beta-Lactoglobulin (beta-Lg), bovine serum albumin, and potato protein isolates. The protein concentration per dry mass in the residual biomass after PEF treatment was increased compared to the control because of the removal of additional nonprotein compounds from the biomass during the extraction process. These results provide currently missing information and technological development for the use of macroalgae as a source of protein for promoting sustainable human nutrition and health.
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