期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1854, 期 7, 页码 798-811出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2014.10.023
关键词
Neuropeptide; Mass spectrometry; Mass spectrometric imaging; Crustacean; Microdialysis; Peptidomics
资金
- National Science Foundation [CHE-1413596]
- National Institutes of Health [1R01DK071801]
- H. I. Romnes Faculty Fellowship
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1413596] Funding Source: National Science Foundation
Neuropeptides represent one of the largest classes of signaling molecules used by nervous systems to regulate a wide range of physiological processes. Over the past several years, mass spectrometry (MS)-based strategies have revolutionized the discovery of neuropeptides in numerous model organisms, especially in decapod crustaceans. Here, we focus our discussion on recent advances in the use of MS-based techniques to map neuropeptides in the spatial domain and monitoring their dynamic changes in the temporal domain. These MS-enabled investigations provide valuable information about the distribution, secretion and potential function of neuropeptides with high molecular specificity and sensitivity. In situ MS imaging and in vivo microdialysis are highlighted as key technologies for probing spatio-temporal dynamics of neuropeptides in the crustacean nervous system. This review summarizes the latest advancement in MS-based methodologies for neuropeptide analysis including typical workflow and sample preparation strategies as well as major neuropeptide families discovered in decapod crustaceans. This article is part of a Special Issue entitled: Neuroproteomics: Applications in Neuroscience and Neurology. (C) 2014 Elsevier B.V. All rights reserved.
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