期刊
TOXINS
卷 11, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/toxins11010056
关键词
simultaneous lateral flow immunoassay; multi-class chemical contaminants; aflatoxin B-1; zearalenone; chlorothalonil; maize and peanut
资金
- National Key R&D Program of China [2018YFC1602505, 2016YFF0201901]
- National Program for support of Top-notch Young Professionals
Multi-class chemical contaminants, such as pesticides and mycotoxins, are recognized as the major risk factors in agro products. It is thus necessary to develop rapid and simple sensing methods to fulfill the on-site monitoring of multi-class chemical contaminants with different physicochemical properties. Herein, a lateral flow immunoassay via time-resolved fluorescence was developed for the rapid, on-site, simultaneous, and quantitative sensing aflatoxin B-1 (AFB(1)), zearalenone (ZEA), and chlorothalonil (CTN) in maize and peanut. The sample preparation was optimized to a single step, combining the grinding and extraction. Under optimal conditions, the sensing method lowered the limits of detection (LOD) to 0.16, 0.52, and 1.21 mu g/kg in maize and 0.18, 0.57, and 1.47 mu g/kg in peanut with an analytical range of 0.48-20, 1.56-200, and 3.63-300 mu g/kg for AFB(1), ZEA and CTN, respectively. The protocol could be completed within 15 min, including sample preparation and lateral flow immunoassay. The recovery range was 83.24-110.80%. An excellent correlation was observed between this approach and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for mycotoxins and gas chromatography-tandem mass spectrometry (GC-MS/MS) for pesticide in maize and peanut. This work could be applied in on-site multi-class sensing for food safety.
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