期刊
ACTA PHARMACOLOGICA SINICA
卷 37, 期 8, 页码 1083-1090出版社
ACTA PHARMACOLOGICA SINICA
DOI: 10.1038/aps.2016.29
关键词
cinnamon; procyanidin oligomers; cinnamtannin B1; procyanidin C1; cinnamtannin D1; diabetes; pancreatic beta-cells; cultured murine islets; lipotoxicity; ROS; insulin secretion
资金
- National Natural Science Foundation [81473262, 21172044]
- National Science and Technology Major Project (Key New Drug Creation and Manufacturing Program) [2012ZX09301001-001, 2013ZX09103001-001]
- Natural Science Foundation of Shanghai, China [15ZR1441200]
- Shanghai Three-year Plan on Promoting TCM Development [ZY3-LCPT-2-1003]
Aim: Cinnamon extracts rich in procyanidin oligomers have shown to improve pancreatic beta-cell function in diabetic db/db mice. The aim of this study was to identify the active compounds in extracts from two species of cinnamon responsible for the pancreatic beta-cell protection in vitro. Methods: Cinnamon extracts were prepared from Cinnamomum tamala (CT-E) and Cinnamomum cassia (CC-E). Six compounds procyanidin B2 (cpd1), (-)-epicatechin (cpd2), cinnamtannin B1 (cpd3), procyanidin C1 (cpd4), parameritannin A1 (cpd5) and cinnamtannin D1 (cpd6) were isolated from the extracts. INS-1 pancreatic beta-cells were exposed to palmitic acid (PA) or H2O2 to induce lipotoxicity and oxidative stress. Cell viability and apoptosis as well as ROS levels were assessed. Glucose-stimulated insulin secretion was examined in PA-treated beta-cells and murine islets. Results: CT-E, CC-E as well as the compounds, except cpd5, did not cause cytotoxicity in the beta-cells up to the maximum dosage using in this experiment. CT-E and CC-E (12.5-50 mu g/mL) dose-dependently increased cell viability in both PA- and H2O2-treated beta-cells, and decreased ROS accumulation in H2O2-treated beta-cells. CT-E caused more prominent beta-cell protection than CC-E. Furthermore, CT-E (25 and 50 mu g/mL) dose-dependently increased glucose-stimulated insulin secretion in PA-treated beta-cells and murine islets, but CC-E had little effect. Among the 6 compounds, trimer procyanidins cpd3, cpd4 and cpd6 (12.5-50 mu mol/L) dose-dependently increased the cell viability and decreased ROS accumulation in H2O2-treated beta-cells. The trimer procyanidins also increased glucose-stimulated insulin secretion in PA-treated beta-cells. Conclusion: Trimer procyanidins in the cinnamon extracts contribute to the pancreatic beta-cell protection, thus to the anti-diabetic activity.
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