期刊
CELL REPORTS
卷 25, 期 1, 页码 259-+出版社
CELL PRESS
DOI: 10.1016/j.celrep.2018.09.007
关键词
-
类别
资金
- Wellcome Trust Research Career Development and Senior Research fellowships [WT088359MA, WT106994MA]
- Korean National Research Foundation (NRF) by the Ministry of Education [2014R1A6A3A03060067]
- MRC grant [MR/N020413/1]
- Hertford College, University of Oxford
- MRC [MR/N020413/1] Funding Source: UKRI
- National Research Foundation of Korea [2014R1A6A3A03060067] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Termination of RNA polymerase II (Pol II) transcription is a key step that is important for 3' end formation of functional mRNA, mRNA release, and Pol II recycling. Even so, the underlying termination mechanism is not yet understood. Here, we demonstrate that the conserved and essential termination factor Seb1 is found on Pol II near the end of the RNA exit channel and the Rpb4/7 stalk. Furthermore, the Seb1 interaction surface with Pol II largely overlaps with that of the elongation factor Spt5. Notably, Seb1 co-transcriptional recruitment is dependent on Spt5 dephosphorylation by the conserved PP1 phosphatase Dis2, which also dephosphorylates threonine 4 within the Pol II heptad repeated C-terminal domain. We propose that Dis2 orchestrates the transition from elongation to termination phase during the transcription cycle by mediating elongation to termination factor exchange and dephosphorylation of Pol II C-terminal domain.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据