期刊
ACS SYNTHETIC BIOLOGY
卷 7, 期 12, 页码 2783-2789出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.8b00392
关键词
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资金
- Commission for the Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI)
- JSPS KAKENHI [15H05897, 17H03621]
- National Bioscience Database Center of JST
- Grants-in-Aid for Scientific Research [17H03621] Funding Source: KAKEN
For many secondary metabolites, heterologous synthesis is the definitive step to determine their required biosynthetic genes. Using a multivector expression system in Saccharomyces cerevisiae, we reconstituted not only two natural statins from two fungal species, i.e., lovastatin from Aspergillus terreus and FR901512 from Xylaria grammica, but also new statin structures by mixing their genes. Combinatorial gene exchange experiments revealed the functional promiscuity of two polyketide synthases in A. terreus, lovB, and lovF; they could synthesize FR901512 with Xylaria genes. Key structure determinants of statins are essential accessory genes that are irreplaceable across species.
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