期刊
ACS SYNTHETIC BIOLOGY
卷 8, 期 2, 页码 358-370出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.8b00393
关键词
miRNA; Chlamydomonas reinhardtii; gene expression; synthetic gene circuit
资金
- OpenPlant from the Biotechnology and Biological Sciences Research Council (BBSRC)
- Engineering and Physical Sciences Research Council (EPSRC)
- BBSRC [BB/L014130/1] Funding Source: UKRI
MicroRNAs (miRNAs), small RNA molecules of 20-24 nts, have many features that make them useful tools for gene expression regulation small size, flexible design, target predictability, and action at a late stage of the gene expression pipeline. In addition, their role in fine-tuning gene expression can be harnessed to increase robustness of synthetic gene networks. In this work, we apply a synthetic biology approach to characterize miRNA-mediated gene expression regulation in the unicellular green alga Chlamydomonas reinhardtii. This characterization is then used to build tools based on miRNAs, such as synthetic miRNAs, miRNA-responsive 3'UTRs, miRNA decoys, and self-regulatory loops. These tools will facilitate the engineering of gene expression for new applications and improved traits in this alga.
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