期刊
ACS SYNTHETIC BIOLOGY
卷 8, 期 2, 页码 232-238出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.8b00383
关键词
isoprenoids; terpenoids; hemiterpene; kinase; lycopene; prenyltransferase
资金
- National Institutes of Health [GM104258]
Isoprenoids are constructed in nature using hemiterpene building blocks that are biosynthesized from lengthy enzymatic pathways with little opportunity to deploy precursor-directed biosynthesis. Here, an artificial alcohol-dependent hemiterpene biosynthetic pathway was designed and coupled to several isoprenoid biosynthetic systems, affording lycopene and a prenylated tryptophan in robust yields. This approach affords a potential route to diverse non-natural hemiterpenes and by extension isoprenoids Modified with non-natural chemical functionality. Accordingly, the prototype chemo-enzymatic pathway is a critical first step toward the construction of engineered microbial strains for bioconversion of simple scalable building blocks into complex isoprenoid scaffolds.
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