期刊
SCIENTIFIC REPORTS
卷 9, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-018-36442-9
关键词
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资金
- Investigator-Initiated Study Program of Janssen Diagnostics, LLC
- S.C.EN.I.C. Network: Department of Medicine and Aging Sciences, School of Medicine and Health Sciences, University G. d'Annunzio of Chieti-Pescara, Chieti, Italy
- BD Biosciences Italia, Milano, Italy
- Department of Transfusion Medicine, Laboratory for Stem Cells Manipulation and Cryopreservation, ASST Spedali Civili di Brescia, Brescia, Italy
- Department of Hematology, Stem Cell Transplantation, Trasfusion Medicine and Cellular Therapy, Campus Bio-Medico University Hospital, Roma, Italy
- Experimental Pharmacology Unit, Istituto Nazionale Tumori Fondazione G. Pascale - IRCCS, Napoli, Italy
- Department of Laboratory Medicine, Clinical Pathology Laboratory, S. Maria degli Angeli Hospital, Pordenone, Italy
- Department of Health Science, University Magna Graecia of Catanzaro, Catanzaro, Italy
Physio-pathologic interrelationships between endothelial layer and graft-versus-host disease (GVHD) have been described leading to assess the entity endothelial GVHD as the early step for clinical manifestations of acute GVHD. The availability of the CellSearch system has allowed us to monitor Circulating Endothelial Cells (CEC) changes in allogeneic hematopoietic stem cell transplantation (allo-HSCT) as useful tool to help clinicians in GVHD diagnostic definition. We have compared CEC counts generated by an ad hoc designed polychromatic-flowcytometry (PFC) Lyotube with those of the CellSearch system. CEC were counted in parallel at 5 timepoints in 50 patients with malignant hematologic disorders undergoing allo-HSCT (ClinicalTrials.gov, NCT02064972). Spearman rank correlation showed significant association between CEC values at all time points (p = 0.0001). The limits of agreement was demonstrated by Bland Altman plot analysis, showing bias not significant at T1, T3, T4, while at T2 and T5 resulted not estimable. Moreover, Passing Bablok regression analysis showed not significant differences between BD Lyotube and CellSearch system. We show that CEC counts, generated with either the CellSearch system or the PFC-based panel, have a superimposable kinetic in allo-HSCT patients and that both counting procedures hold the potential to enter clinical routine as a suitable tool to assist clinicians in GVHD diagnosis.
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