期刊
NATURE COMMUNICATIONS
卷 9, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-018-06727-8
关键词
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资金
- National Institute of Health [R01AI118502, R21HD092828, P30ES000002]
- Harvard T. H. Chan School of Public Health, Harvard Center for Environmental Health through Harvard NIEHS Grant
- American Board of Obstetrics and Gynecology
- American College of Obstetricians and Gynecologists
- American Society for Reproductive Medicine
- Society for Reproductive Endocrinology and Infertility through ASRM Award
- Harvard University Center for AIDS Research (CFAR) [5P30AI060354-14]
HIV-1 infection is a major health threat in both developed and developing countries. The integration of mobile health approaches and bioengineered catalytic motors can allow the development of sensitive and portable technologies for HIV-1 management. Here, we report a platform that integrates cellphone-based optical sensing, loop-mediated isothermal DNA amplification and micromotor motion for molecular detection of HIV-1. The presence of HIV-1 RNA in a sample results in the formation of large-sized amplicons that reduce the motion of motors. The change in the motors motion can be accurately measured using a cellphone system as the biomarker for target nucleic acid detection. The presented platform allows the qualitative detection of HIV-1 (n = 54) with 99.1% specificity and 94.6% sensitivity at a clinically relevant threshold value of 1000 virus particles/ml. The cellphone system has the potential to enable the development of rapid and low-cost diagnostics for viruses and other infectious diseases.
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