期刊
NATURE COMMUNICATIONS
卷 9, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-018-07235-5
关键词
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资金
- UK Medical Research Council [MR/N013204/1, MR/026993/1]
- Sir Christopher Benson Studentship (University of Southampton)
- Wessex Institute of Virology and Infectious Disease
- Southampton NIHR Biomedical Research Centre
- Wellcome Trust
- MRC [MR/N013204/1, MR/R015600/1, MR/N026993/1] Funding Source: UKRI
Neisseria lactamica is a harmless coloniser of the infant respiratory tract, and has a mutuallyexcluding relationship with the pathogen Neisseria meningitidis. Here we report controlled human infection with genomically-defined N. lactamica and subsequent bacterial microevolution during 26 weeks of colonisation. We find that most mutations that occur during nasopharyngeal carriage are transient indels within repetitive tracts of putative phasevariable loci associated with host-microbe interactions (pgl and Igt) and iron acquisition (fetA promotor and hpuA). Recurrent polymorphisms occurred in genes associated with energy metabolism (nuoN, rssA) and the CRISPR-associated cast. A gene encoding a large hypothetical protein was often mutated in 27% of the subjects. In volunteers who were naturally co-colonised with meningococci, recombination altered allelic identity in N. lactamica to resemble meningococcal alleles, including loci associated with metabolism, outer membrane proteins and immune response activators. Our results suggest that phase variable genes are often mutated during carriage-associated microevolution.
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