期刊
NATURE COMMUNICATIONS
卷 10, 期 -, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-018-07885-5
关键词
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资金
- Spanish Ministry of Economy and Competitiveness [BFU2014-55090-P]
- Centro de Excelencia Severo Ochoa 2013-2017
- Centro de Excelencia Maria de Maeztu 2016-2019
- CERCA Programme of the Generalitat de Catalunya
- European Regional Development Fund (ERDF)
- FPI fellowship
- MINECO/FEDER, UE [BFU2014-55090-P, BFU2017-86471-P]
- Deutsche Forschungsgemeinschaft (DFG) [KU 3467/1-1]
- Howard Hughes International Early Career
- Obra Social La Caixa
- Secretaria d'Universitats i Recerca del Departament d'Economia i Coneixement de la Generalitat de Catalunya
- MICINN [FJCI-2016-29558]
- Spanish Ministry of Economy, Industry and Competitiveness (MEIC)
- Generalitat de Catalunya through the Departament de Salut
- Departament d'Empresa i Coneixement
- [U01 MH106874]
Mammalian Y chromosomes are often neglected from genomic analysis. Due to their inherent assembly difficulties, high repeat content, and large ampliconic regions, only a handful of species have their Y chromosome properly characterized. To date, just a single human reference quality Y chromosome, of European ancestry, is available due to a lack of accessible methodology. To facilitate the assembly of such complicated genomic territory, we developed a novel strategy to sequence native, unamplified flow sorted DNA on a MinION nanopore sequencing device. Our approach yields a highly continuous assembly of the first human Y chromosome of African origin. It constitutes a significant improvement over comparable previous methods, increasing continuity by more than 800%. Sequencing native DNA also allows to take advantage of the nanopore signal data to detect epigenetic modifications in situ. This approach is in theory generalizable to any species simplifying the assembly of extremely large and repetitive genomes.
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