期刊
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
卷 11, 期 5, 页码 429-437出版社
SPRINGER
DOI: 10.1007/s12265-018-9824-y
关键词
Cardiac mesenchymal stem cells; Exosomes; Hind limb ischemia; Angiogenesis; miR-7116-5p
资金
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL134354, R01HL086555] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR070029] Funding Source: NIH RePORTER
- NHLBI NIH HHS [R01 HL134354, R01 HL086555] Funding Source: Medline
- NIAMS NIH HHS [R01 AR070029] Funding Source: Medline
We demonstrated the effects of exosomes secreted by cardiac mesenchymal stem cells (C-MSC-Exo) in protecting acute ischemic myocardium from reperfusion injury. To investigate the effect of exosomes from C-MSC on angiogenesis, we injected C-MSC-Exo or PBS intramuscularly into ischemic hind limb. Blood perfusion of limb was evaluated by laser Doppler Imaging. We observed that ischemic limb treated with C-MSC-Exo exhibits improved blood perfusion compared to ischemic limb treated with PBS at 2 weeks and 1 month after induction of limb ischemia. To explore the potential mechanisms underlying C-MSC-Exo's angiogenetic effect, we performed microRNA array analysis and identify mmu-miR-7116-5p as the most abundant enriched miRNA detected in C-MSC-Exo. Bioinformatics' analysis shows that miR-7116-5p negatively regulates protein polyubiquitination. In conclusion, our study demonstrated that intramuscular delivery of C-MSC-Exo after limb ischemia improves blood perfusion, and we identified the most abundant miRNAs that are preferentially enriched in C-MSC-Exo.
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