期刊
BIOANALYSIS
卷 10, 期 20, 页码 1691-1704出版社
FUTURE SCI LTD
DOI: 10.4155/bio-2018-0156
关键词
biotherapeutics; chromatography; HPLC; LC-MS; proteins; UHPLC; UPLC
资金
- Indiana Space Grant Consortium-National Aeronautics Space Administration (INSGC-NASA)
- National Science Foundation (NSF-MRI) [1531360]
Ultrahigh performance liquid chromatography (UHPLC) uses small stationary-phase particle size (<2m) and high pressure in order to achieve rapid and efficient separations. The speed and high resolution of this method has made it a valuable tool for analyzing the complex glycosylation patterns found in post-translationally modified proteins. This article highlights the differences between UHPLC and HPLC and reviews recent UHPLC applications and developments for detecting glycosylated proteins (e.g., glycomics studies) and characterizing glycosylated pharmaceuticals (e.g., monoclonal antibodies).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据