期刊
VIRUSES-BASEL
卷 10, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/v10110597
关键词
flavivirus; Zika virus (ZIKV); reverse genetics; infectious clone; full-length molecular clone; bacterial artificial chromosome; replicon; infectious RNA
类别
资金
- Spanish Ministry of Economy and Competitiveness (MINECO) [BFU2016-79127-R]
- National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) [R21 AI130500]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R21AI130500] Funding Source: NIH RePORTER
Zika virus (ZIKV) is an emergent mosquito-borne member of the Flaviviridae family that was responsible for a recent epidemic in the Americas. ZIKV has been associated with severe clinical complications, including neurological disorder such as Guillain-Barre syndrome in adults and severe fetal abnormalities and microcephaly in newborn infants. Given the significance of these clinical manifestations, the development of tools and reagents to study the pathogenesis of ZIKV and to develop new therapeutic options are urgently needed. In this respect, the implementation of reverse genetic techniques has allowed the direct manipulation of the viral genome to generate recombinant (r)ZIKVs, which have provided investigators with powerful systems to answer important questions about the biology of ZIKV, including virus-host interactions, the mechanism of transmission and pathogenesis or the function of viral proteins. In this review, we will summarize the different reverse genetic strategies that have been implemented, to date, for the generation of rZIKVs and the applications of these platforms for the development of replicon systems or reporter-expressing viruses.
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