期刊
VETERINARY MICROBIOLOGY
卷 227, 期 -, 页码 103-111出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2018.10.031
关键词
Mastitis; Streptococcus uberis; Signaling pathway; Inflammation; ROS
资金
- National Natural Science Foundation of China [31472164, 31672515]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Mammary epithelial cells (MECs) play an important role in debating Streptococcus uberis (S. uberis) infection. Toll like receptor (TLR) engagement leads to the recruitment of phosphatidylinositol 3 kinases (PI3K). In order to investigate the relationship of TLRs/NF-kappa B and PI3K/Akt/mTOR signaling pathways in S. uberis infection in MECs, we challenged MECs (EpH4-Ev) with S. uberis 0140 J and quantified the adaptor molecules in these two signaling pathways, as-well-as proinflammatory cytokines and cell damage. The results indicate that the host's responses to virulent S. uberis infection are complex. In MECs, both TLR2 and TLR4 are detecting S. uberis infection and TLR2 is the principal receptor. The role of the PI3K/Akt/mTOR pathway in inflammatory regulation is independent of the activation of TLRs/NF-kappa B. Cross-talk between PI3K/Akt/mTOR and TLRs/NF-kappa B signaling pathways promote inflammation. This study increases our understanding of the molecular defense mechanisms of MECs in S. uberis mastitis, and provides theoretical support for the prevention of this disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据