期刊
ACTA NEUROPATHOLOGICA
卷 131, 期 5, 页码 725-736出版社
SPRINGER
DOI: 10.1007/s00401-016-1555-z
关键词
Lymphatic drainage; Basement membranes; Capillaries; Arteries; Amyloid-beta; Nanoparticles neuroimmunology; Alzheimer's disease; Virchow-Robin space; Drug delivery
资金
- Biotechnology and Biological Sciences Research Council [BB/K015540/1]
- Age UK [PhD-368]
- Biogen
- BBSRC [BB/K015540/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/K015540/1] Funding Source: researchfish
In the absence of conventional lymphatics, drainage of interstitial fluid and solutes from the brain parenchyma to cervical lymph nodes is along basement membranes in the walls of cerebral capillaries and tunica media of arteries. Perivascular pathways are also involved in the entry of CSF into the brain by the convective influx/glymphatic system. The objective of this study is to differentiate the cerebral vascular basement membrane pathways by which fluid passes out of the brain from the pathway by which CSF enters the brain. Experiment 1: 0.5 A mu l of soluble biotinylated or fluorescent A beta, or 1 A mu l 15 nm gold nanoparticles was injected into the mouse hippocampus and their distributions determined at 5 min by transmission electron microscopy. A beta was distributed within the extracellular spaces of the hippocampus and within basement membranes of capillaries and tunica media of arteries. Nanoparticles did not enter capillary basement membranes from the extracellular spaces. Experiment 2: 2 A mu l of 15 nm nanoparticles were injected into mouse CSF. Within 5min, groups of nanoparticles were present in the pial-glial basement membrane on the outer aspect of cortical arteries between the investing layer of pia mater and the glia limitans. The results of this study and previous research suggest that cerebral vascular basement membranes form the pathways by which fluid passes into and out of the brain but that different basement membrane layers are involved. The significance of these findings for neuroimmunology, Alzheimer's disease, drug delivery to the brain and the concept of the Virchow-Robin space are discussed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据