期刊
STRUCTURE
卷 27, 期 2, 页码 344-+出版社
CELL PRESS
DOI: 10.1016/j.str.2018.10.003
关键词
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资金
- Helen Hay Whitney Foundation postdoctoral fellowship
- National Science Foundation [STC-1231306]
- National Institutes of Health [DP2EB020402, GM063210]
- Searle Scholar
- Pew Scholar
- Amgen Young Investigator
- Packard Fellow
- [1-S1OD021634]
There does not currently exist a standardized indicator of how well cryo-EM-derived models represent the density from which they were generated. We present a straightforward methodology that utilizes freely available tools to generate a suite of independent models and to evaluate their convergence in an EM density. These analyses provide both a quantitative and qualitative assessment of the precision of the models and their representation of the density, respectively, while concurrently providing a platform for assessing both global and local EM map quality. We further use standardized datasets to provide an expected deviation within a suite of models refined against EM maps reported to be at 5 angstrom resolution or better. Associating multiple atomic models with a deposited EM map provides a rapid and accessible reporter of convergence, a strong indicator of highly resolved molecular detail, and is an important step toward an FSC-independent assessment of map and model quality.
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