4.7 Article

BIN1 Induces the Formation of T-Tubules and Adult-Like Ca2+ Release Units in Developing Cardiomyocytes

期刊

STEM CELLS
卷 37, 期 1, 页码 54-64

出版社

WILEY
DOI: 10.1002/stem.2927

关键词

hESC; Cardiac myocytes; BIN1; T-tubules; Ca(V)1.2; Calcium release units

资金

  1. U.S. National Institutes of Health (NIH) [R01-HL085686, 1K99AG056595-01]
  2. American Heart Association [15SDG25560035, 16SDG30970046]

向作者/读者索取更多资源

Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) are at the center of new cell-based therapies for cardiac disease, but may also serve as a useful in vitro model for cardiac cell development. An intriguing feature of hESC-CMs is that although they express contractile proteins and have sarcomeres, they do not develop transverse-tubules (T-tubules) with adult-like Ca2+ release units (CRUs). We tested the hypothesis that expression of the protein BIN1 in hESC-CMs promotes T-tubules formation, facilitates Ca(V)1.2 channel clustering along the tubules, and results in the development of stable CRUs. Using electrophysiology, [Ca2+](i) imaging, and super resolution microscopy, we found that BIN1 expression induced T-tubule development in hESC-CMs, while increasing differentiation toward a more ventricular-like phenotype. Voltage-gated Ca(V)1.2 channels clustered along the surface sarcolemma and T-tubules of hESC-CM. The length and width of the T-tubules as well as the expression and size of Ca(V)1.2 clusters grew, as BIN1 expression increased and cells matured. BIN1 expression increased Ca(V)1.2 channel activity and the probability of coupled gating within channel clusters. Interestingly, BIN1 clusters also served as sites for sarcoplasmic reticulum (SR) anchoring and stabilization. Accordingly, BIN1-expressing cells had more Ca(V)1.2-ryanodine receptor junctions than control cells. This was associated with larger [Ca2+](i) transients during excitation-contraction coupling. Our data support the view that BIN1 is a key regulator of T-tubule formation and Ca(V)1.2 channel delivery. By studying the role of BIN1 during the differentiation of hESC-CMs, we show that BIN1 is also important for Ca(V)1.2 channel clustering, junctional SR organization, and the establishment of excitation-contraction coupling. Stem Cells 2019;37:54-64

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