Synthesis and spectral analysis of fluorescent probes for Ce4+ and OCl- ions based on fluorescein Schiff base with amino or hydrazine structure: Application in actual water samples and biological imaging

Two Schiff base fluorescein probes (FDA, FDH) based on fluorescein-aldehyde and nitroaniline derivatives were synthesized. The effects of amino and hydrazine substituents in fluorescein backbones were examined via fluorescence and absorbance spectra. In the presence of Ce4+, the fluorescence of FDA was quenched due to the ligand to metal charge transfer (LMCT). Hypochloric acid can react with the C=N bond, and blocking the photo induced electron transfer (PET) of FDH leads to enhancement of the fluorescence. FDA showed detection limits for Ce4+ and OCl- as low as 63 nM in concentration range of 0-4 mu M. FDH showed detection limits for OCl- as low as 0.8 mu Min concentration rang 0-100 mu M. Polyvinylidene fluoride (PVDF) membrane containing the probes was prepared for the real-time qualitative detection of Ce4+ and OCl- in real water samples. The probes were successfully applied to biological imaging in vascular smooth muscle cells (VSMCs) and are expected to find applications in biosensing. (C) 2019 Elsevier B.V. All rights reserved.