4.7 Article

Proximity ligation assay induced and DNAzyme powered DNA motor for fluorescent detection of thrombin

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2018.08.062

关键词

Aptamer; DNA motor; Signal amplification; Thrombin; Proximity ligation assay

资金

  1. Chongqing Key Laboratory of Catalysis and New Environmental Materials [KFJJ2017033]
  2. Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences [201712]
  3. Science and Technology Research Program of Chongqing Municipal Education Commission [KJ1706156]
  4. Project of Wenzhou Science &Technology Bureau [W20170006]
  5. Open Project of State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials [17kffk06]
  6. Startup Foundation of Chongqing Technology and Business University [1756001]
  7. National Natural Science Foundation of China [21601164, 31300819]
  8. [CQCM-2016-05]

向作者/读者索取更多资源

A novel DNA motor for thrombin detection was described here based on proximity ligation assay (PLA) induced DNAzyme recycling cleavage. Fluorophore labeled DNA is modified on gold nanoparticles (AuNPs) and the fluorescent signal is quenched by AuNPs. The PLA between target thrombin and two aptamers induces the forming of Mg2+-dependent DNAzyme. The fluorophore labeled DNA is cleaved circularly by the DNAzyme, releasing the fluorescent fragment from AuNPs surface. The cleavage and rebinding process create a processive walking along AuNPs surface track. As a result, the fluorescent intensity recovers significantly. A good linear relationship is obtained between the ratio of fluorescence intensity and thrombin concentration in the range from 10 pM to 10 nM. The limit of detection is calculated to be 4 pM. These results are comparable or even better than other amplification based methods. (C) 2018 Elsevier B.V. All rights reserved.

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