期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 207, 期 -, 页码 150-155出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2018.09.022
关键词
Fluorescent probe; Hydrogen sulfide; Ratiometric signal; Dual nucleophilic reaction; Cell imaging
类别
资金
- National Natural Science Foundation of China [21601111]
- Natural Science Foundation of Shanxi Province [201601D011017, 201701D221029]
- Scientific Activities of Selected Returned Overseas Professionals in Shanxi Province [2015287]
- Shanxi Scholarship Council of China [2017-011]
Hydrogen sulfide (H2S) can undergo dual nucleophilic reaction, which is a wise and effective way to distinguish biothiols and H2S. A novel H2S fluorescence probe, 4-{2-[4-(2-disulfide pyridyl-benzoyloxy)-phenyl]-vinyl}-1-methyl-pyridinium[e]iodide (DSPBP), with two nucleophilic reaction sites has been developed. The spectra results showed that DSPBP could detect H2S in ratiometric and colorimetric signals and has excellent selectivity and sensitivity. The fluorescence ratiometric signals (F-520/F-450) displayed a prominent increase from 0.74 to 7.08, the fluorescence color turned to yellow form blue simultaneously. The linear range was 2-14 mu M and its detection limit was 25.7 nM. Moreover, the biocompatibility of DSPBP was fine and its toxicity was very low. It has been successfully used for imaging H2S in cells. (C) 2018 Elsevier B.V. All rights reserved.
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