期刊
SENSORS
卷 18, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/s18124298
关键词
Optical fiber biosensors; fiber Bragg gratings (FBG); refractive index sensor; aptamer; thrombin; biosensor
资金
- project of Nazarbayev University: LIFESTART (Lab-in-a-fiber for smart thermo-haptic treatment of tumors)
- project of Nazarbayev University: FOSTHER (ORAU projects)
- Social Policy Grant-Nazarbayev University
- Research and development of the new Nano-Optical Sensor based on Polymer Optical Fiber for Near-Field Scanning Optical Microscopy project
A biosensor based on an etched Fiber Bragg Grating (EFBG) for thrombin detection is reported. The sensing system is based on a Fiber Bragg Grating (FBG) with a Bragg wavelength of 1550 nm, wet-etched in hydrofluoric acid (HF) for similar to 27 min, to achieve sensitivity to a refractive index (RI) of 17.4 nm/RIU (refractive index unit). Subsequently, in order to perform a selective detection of thrombin, the EFBG has been functionalized with silane-coupling agent 3-(aminopropyl) triethoxysilane (APTES) and a cross-linker, glutaraldehyde, for the immobilization of thrombin-binding aptamer. The biosensor has been validated for thrombin detection in concentrations ranging from 10 nM to 80 nM. The proposed sensor presents advantages with respect to other sensor configurations, based on plasmonic resonant tilted FBG or Long Period Grating (LPG), for thrombin detection. Firstly, fabricating an EFBG only requires chemical etching. Moreover, the functionalization method used in this study (silanization) allows the avoidance of complicated and expensive fabrications, such as thin film sputtering or chemical vapor deposition. Due to their characteristics, EFBG sensors are easier to multiplex and can be used in vivo. This opens new possibilities for the detection of thrombin in clinical settings.
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