期刊
SCIENTIA HORTICULTURAE
卷 243, 期 -, 页码 320-326出版社
ELSEVIER
DOI: 10.1016/j.scienta.2018.08.055
关键词
Abiotic stress; Amur grape; Antioxidant enzyme; Cold stress; Low temperature
类别
资金
- Youth Innovation Promotion Association of CAS [2015281]
- China Postdoctoral Science Foundation [2016M601166]
- Grape Breeding Project of Ningxia [NXNYYZ201502]
- Open Project Program of the Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology (Fujian Agriculture and Forestry University)
Low temperature is a vital environmental factor that affects plant growth, development and crop yields. Ethylene response factor (ERF) is a plant specific transcription factor with important roles in stress adaptation. However, the regulatory mechanism on how grapevine responds to cold stress is still unclear. In this study, we isolated and characterized two ERF genes from Vitis amurensis designated as VaERF080 and VaERF087. The function of these two genes was investigated in transgenic Arabidopsis. We found that both VaERF080 and VaERF087 were induced by cold stress and ethylene. The overexpression of these genes in Arabidopsis resulted in enhanced cold tolerance. The VaERF080- and VaERF087-overexpressing (OE) Arabidopsis lines showed lower levels of malondialdehyde (MDA) content and higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) under cold stress compared to wild-type (WT) Arabidopsis. Nine representative cold-responsive genes, CBF1, CBF2, ICE1, ZAT12, KIN1, SIZ1, RD29A, COR15A, and COR47, were significantly upregulated in OE Arabidopsis. These results revealed that both VaERF080 and VaERF087 may improve the cold tolerance of transgenic Arabidopsis through increasing antioxidant enzyme activities and regulating the expression of cold-related genes.
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